Rapid differentiation of Mycobacterium tuberculosis and M. bovis by high-resolution melt curve analysis.
نویسندگان
چکیده
Identification and characterization of the Mycobacterium tuberculosis strains are important for clinical and therapeutic management of tuberculosis. Real-time PCR with a high-resolution melt assay was found to improve the diagnostic process. The assay includes differentiation between M. tuberculosis and Mycobacterium bovis based on one single-nucleotide polymorphism (SNP) in the narGHJI and oxyR genes and determination of M. bovis based on the region of differences 1 (RD1). This assay correctly identified the 7 tested Mycobacterium reference strains and 52 clinical samples with a sensitivity of 2 pg DNA. This assay will help in prescribing adequate treatment and monitoring disease dynamics.
منابع مشابه
Rapid differentiation of Mycobacterium tuberculosis and M. bovis
45 46 Identification and characterization of the Mycobacterium tuberculosis strains is important for 47 clinical and therapeutic management of tuberculosis. Real time PCR with high-resolution melt 48 assay were found to improve the diagnostic process. The assay includes differentiation between 49 M. tuberculosis and M. bovis based on one SNP in the narGHJI and oxyR genes, and 50 determination o...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 48 11 شماره
صفحات -
تاریخ انتشار 2010